种属:人
样品类型:细胞,组织等
PCR ARRAY介绍
PCR ARRAY又称PCR芯片,运用高通量荧光定量PCR方法,在一张96孔或384孔板上同时对某个信号通路或疾病相关基因的表达量变化进行检测,芯片上的基因包括了与研究对象有确定关系的基因或者待考证的基因;目前启因生物针对不同的信号通路和疾病相关基因设计了150多款功能分类PCR芯片,涵盖生命科学研究的各个领域。
人应激和毒性通路发现PCR芯片介绍
启因生物的人应激和毒性通路发现ARRAY含有84个与人应激和毒性通路发现相关的基因,包括氧化应激、细胞死亡、DNA损伤、细胞周期检测点等相关基因等。
基因列表
Oxidative Stress: FTH1, GCLC, GCLM, GSR, GSTP1, HMOX1, NQO1, PRDX1, SQSTM1, TXN, TXNRD1.
Hypoxia: ADM, ARNT, BNIP3L, CA9, EPO, HMOX1, LDHA, MMP9 (Gelatinase B), SERPINE1 (PAI-1), SLC2A1, VEGFA.
Osmotic Stress: AKR1B1, AQP1, AQP2, AQP4, CFTR, EDN1, HSPA4L, NFAT5, SLC5A3.
Cell Death:
Apoptosis: CASP1 (ICE), FAS, MCL1, TNFRSF10A, TNFRSF10B (DR5), TNFRSF1A.
Autophagy: ATG5, ATG7, ATG12, BECN1, FAS, ULK1.
Necrosis: FAS (TNFRSF6), GRB2, PARP1 (ADPRT1), PVR, RIPK1, TNFRSF10A, TNFRSF1A, TXNL4B.
Inflammatory Response: CCL2 (MCP-1), CD40LG (TNFSF5), CRP, IFNG, IL1A, IL1B, IL6, IL8, TLR4, TNF.
DNA Damage Signaling:
Cell Cycle Checkpoint/Arrest: CDKN1A (p21CIP1/WAF1), CHEK1, CHEK2 (RAD53), DDIT3 (GADD153/CHOP), HUS1, MRE11A, NBN (NBS1), RAD17, RAD9A.
Other Responses: ATM, ATR, DDB2, GADD45A, GADD45G, RAD51, TP53, XPC.
Heat Shock Proteins/Unfolded Protein Response: ATF4, ATF6, ATF6B, BBC3, BID, CALR, DDIT3, DNAJC3, HSP90AA1, HSP90B1 (TRA1), HSPA4 (HSP70), HSPA5 (GRP78).
参考文献
Zhu J J, Liu Y F, Zhang Y P, et al. VAMP3 and SNAP23 mediate the disturbed flow-induced endothelial microRNA secretion and smooth muscle hyperplasia[J]. Proceedings of the National Academy of Sciences, 2017, 114(31): 8271-8276. (IF9.661)
Zheng H S, Guo W Q, Wu Q L, et al. Electro-peroxone pretreatment for enhanced simulated hospital wastewater treatment and antibiotic resistance genes reduction[J]. Environment international, 2018, 115: 70-78. (IF7.088)
Pu C, Liu H, Ding G, et al. Impact of direct application of biogas slurry and residue in fields: In situ analysis of antibiotic resistance genes from pig manure to fields[J]. Journal of Hazardous Materials, 2018, 344: 441-449.(IF6.065)
样品类型 | 处理方法 | 送样量 |
贴壁细胞 | ①倒出培养液,用 1×PBS 清洗一次。 | 至少1×106个细胞 |
悬浮细胞 | ①将悬浮培养细胞连同培养液一起倒入离心管中,8,000 g 4℃离心2 分钟,弃上清。 | 至少1×106个细胞 |
组织(动物器官类) | 离心管中加1 ml 的 trizol,确保浸没组织,-80℃保存 | 至少20mg,约小黄豆大小 |